Microbial/yeast fermentation

LuinaBio can use Saccharomyces cerevisiae, a species of yeast, in fermentation to produce biopharmaceuticals. The advantages of using S. cerevisiae as a cell factory for the production of biopharmaceuticals is that there is a tremendous amount of information available about the organism. This provides an extensive knowledge base for further engineering.

We also offer clients the ability to produce biopharmaceuticals using Pichia pastoris, a robust yeast expression system that produces high levels of recombinant proteins. The Pichia system is stable, durable and cost-effective. Pichia grows on simple media and secretes low amounts of endogenous protein, making it easier to recover and purify the desired recombinant protein from the cell supernatant.

Pichia has two primary advantages of S. cerevisiae in lab and industrial settings. A methylotroph, Pichia can grow with the simple alcohol methanol as its only source of energy, a system that’s cheap to set up and maintain. Pichia can additionally grow in media containing only one carbon source and one nitrogen source and grow to very high cell densities, a great advantage when trying to produce large quantities of protein without expensive equipment.

Microbial manufacturing is underpinned by fermentation capability up to 500L scale. We are able to offer a full suite of manufacturing both whole cell preparations, recombinant products from microbial hosts and naturally produced cellular products such as carbohydrates. Processing can be undertaken under GMP or non GMP as needed.

We can undertake:
– Master and working cell bank preparation, validation and storage
– Fermentation and subsequent downstream purification
– Up and downscale work up optimisation
– Downstream processing optimisation and development